Different tools have been developed to test organisational citizenship behaviour (OCB). However, psychometric properties developed and tested in a particular context do not guarantee their fit in all contexts and especially the operationalisation and application of dimensions of OCB may differ from country to country. Accordingly, in this study we set out to test the psychometric properties of Podsakoff et al.’s (1990) measure of OCB in the context of academic staff in selected universities in Uganda.Taking a positivism strand, we used a self-administered questionnaire and collected data from a sample of 159 academic staff drawn from MakerereUniversity (Mak), Mbarara University of Science and Technology (MUST), and Mountains of the Moon University (MMU), who responded to a self-administered questionnaire on the instrument. Our analysis involved applying confirmatory factor analysis (CFA) retesting the reliabilities ofthe constructs using the Cronbach’s alpha method and Pearson’s linear correlations to check construct relatedness. We found that all the five constructs in the shortened version were valid, and that the five constructs were, however, interrelated. Based on Podsakoff et al.’s (1990) instrument,this research confirms the scale in the higher education context. We conclude that Podsakoff et al.’s (1990) instrument is valid and reliable. Nevertheless,we recommend other researchers to continue testing it in different contexts with the intent of refining it and also to have a sample size bigger than ours if the best results are to be obtained.