The main purpose of this paper was to analyse the in which entrepreneurial culture can be infused in universities. The study adopted the intrapreneurship theory. Universities are being required to operate more entrepreneurially, commercialising the outcomes of their research and spinning out new knowledge-based enterprises. Against this background, the study explored the culture of entrepreneurialism at Kyambogo University, in the Faculty of Engineering. The study attempted to answer the question of how the entrepreneurial culture among academic staff in universities can enhance entrepreneurship. The study adopted a descriptive case study design. Data was collected through one-on-one interview, document analysis and site visit and analysed thematically. The findings show that the existing entrepreneurial elements, such as the promotion of a close relationship with the business community and the promotion of teaching of entrepreneurship, are loosely coupled with one another, and that there is an infant entrepreneurial culture at the faculty. Additionally,the study uncovered a number of impediments to the deepening of an entrepreneurial culture, including bureaucratic procedures, limited incentives, and limited financial and technical support for innovative ideas. It was concluded that: Kyambogo University policies are not fully activated to steer entrepreneurship; infrastructural units at the university lack the bargaining strength to negotiate official licensing contracts with industry; and less attention has been given to deepening analysis of how entrepreneurship is mainstreamed in the university. The study recommended that university management should ensure that entrepreneurial activities are fully integrated into all programmes and actions of the university. The main contribution of this paper is that the findings may help universities to design policies that can stimulate entrepreneurial activities.